Nanobiotechnologytechnology

Nanobiotechnologytechnology allows the delivery of the drug in the right place at the right time

The medical applications of Nanobiotechnologytechnology are very promising because of the possibility offered by miniaturization, and the interaction in a targeted manner with biological tissues, cells or molecules. The most important pharmaceutical applications of Nanotechnology are in drug delivery. Apart from offering a solution to solubility problems, Nanobiotechnologytechnology provides many intracellular delivery possibilities. For example, the Nanobiotechnology vector containing ligands could deliver drugs to the interior of the cell by a process called receptor mediated endocytosis.

Nanoparticles are defined as particles with size below 1 µm and these can consist of different biodegradable materials like natural or synthetic polymers, lipids or phospholipids. The ability of nanoencapsulating a drug provides further protection against lytic agents, thus prolonging the life of the drug in vivo. The other main advantage is the ability of nanoparticles to target tumor cells and cross membrane barriers such as the blood brain barrier (BBB). The other advantage of nanoparticle targeting is the reduction in toxicity due to non specific binding. Targeted therapies "home in" on the cancer cell while sparing healthy cells. In the age of genetic manipulation and somatic gene therapy, transfection systems use nanoparticles which are custom tailored for specific applications.

Nanotechnology Formulation

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At BioVolutions we synthesize biodegradable nanoparticles for targeting diseases such as cancer or viral infections. We encapsulate your drug and test its targeting potential using in vitro systems Such as the A375 melanoma in vitro model). Your drug may consist of a biological molecule, a synthetic peptide or a small molecule. We also have extensive experience in immobilizing proprietary peptides and antibodies on the surface to confer targeting specificity to the nanoparticulate system. All this work is done under strict confidentiality and to benefit the IP of the client.

For imaging application BioVolutions offers the coumarin-loaded nano / microspheres shown in the table below from stock. BioVolutions will support any Nanotechnology development to your specification.

Example of application

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Figure 1.	Figure 2.
Figure 1. This Scanning Electron Micrograph (SEM) shows biopolymer nano and microparticles obtained using the emulsification apparatus as shown in Figure 2. As you can see from the SEM picture the particle size distribution is quite large, as is common with this type of equipment. Narrowing of the size distribution can be obtained by the use of surface active agents or by using membrane emulsification systems.
Figure 3.	Figure 4.
Figure 3. Narrowing of the particle size distribution is achieved by using higher pressure approaching 10,000 psi. In this SEM, PLGA nanoparticles of size around 100-200 nanometers are obtained using a Microfluidizer apparatus as shown in Figure 4.
Figure 5.
Figure 5. Unilamellar Liposomes obtained using the Microfluidizer apparatus as shown in Figure 4.